Aims and Scope
Dosed Exposure to Low Temperature as a Breeding Background in The Selection of Gene Pool Breeds of Chickens for Viral Vaccines ProductionOlga I. Stanishevskaya, Elena S. Fedorova
One of the ways to utilize the potential of local breeds is to use them as producers of raw materials for the bio-industry (production of vaccines and diagnostics for animals and humans).
Breeding of Russian White (PRWC) laying hens in 5 generations was carried out with the aim of increasing the output of allantois-amniotic fluid of embryos with a selective background (cooling of hatching eggs during the sensitive period of embryogenesis). In F5, the thermoregulation capabilities of 7-day-old PRWC chickens were studied in comparison with Amrox chickens under the influence of a daily stress factor (cooling at +10oC for 30 minutes) and raising at +22oC. The level of allantois-amniotic fluid output and biological activity of the Newcastle disease virus in F5 PRWC embryos in comparison with F0 and commercial line embryos was evaluated.
Day-old PRWC chicks responded to exposure to low sublethal temperature with muscle shivering, but, unlike Amrox chickens, they were active and retained the reflex of searching for food. PRWC chickens raised at low temperatures up to 7 days of age maintained the same growth rate as chickens raised at a common regime but used less residual yolk, which practically did not decrease the body temperature, and change the level of thyrotrophic hormone in response to the dosed exposure at low temperatures at 7 days of age. The content of doses of the Newcastle disease virus (EID50) in the extraembryonic fluid of F5-embryos of PRWC was 32.3 times higher than that of commercial line ones.
PRWC chicks in the early neonatal period are more reactive at low temperature and have better thermoregulation mechanisms. PRWC embryos can be recommended for use in the production of various viral vaccines as well as diagnosticums.
December 31, 2020
- December 22, 2020
- December 15, 2020
- November 27, 2020
- November 27, 2020
- October 15, 2020
- October 15, 2020
Induction of Bacterial Canker Resistance in Tomato Plants Using Plant Growth Promoting RhizobacteriaYuliya Kolomiiets, Ivan Grygoryuk, Artur Likhanov, Lyudmila Butsenko, Yaroslav Blume
By inducing the production of inhibitory allelochemicals and mechanisms of systemic resistance Plant Growth Promoting Bacteria (PGPB) help plants to cope with stresses.
Materials and Methods:
In this study cell suspensions of Bacillus subtilis, Pseudomonas fluorescens or Azotobacter chroococcum were used to test the efficacy of these PGPB in inducing resistance in tomato (Lycopersicon esculentum Mill) against Clavibacter michiganensis subsp michiganensis, a bacteria known to cause canker disease. To test this hypothesis, seedlings of Chaika variety, characterized by short growing, early-ripening, high productivity and resistance against fusarium and the C. michiganensis strain ІZ-38 isolated in Kyiv were employed.
Results and Conclusion:
The use of cell suspensions of the PGPB B. subtilis, A. chroococcum or P. fluorescens induced an increment in the resistance of tomato plants against the causative agent of bacterial canker (C. michiganensis subsp. michiganensis) by 42–50%. PGPB in fact promoted in C. michiganensis infected tomato plants: i) the accumulation of chlorophyll a and b and carotenoids; ii) the thickening of the upper and lower epidermis of leaves; iii) the deposition of biopolymers with protective properties in epidermal cells; iv) the activity of the peroxidase enzyme and v) the net productivity of photosynthesis.
December 31, 2019
- September 30, 2019
- March 26, 2019
- February 28, 2019
- May 31, 2018
- February 27, 2018
- April 21, 2017