RESEARCH ARTICLE
Biobutanol Production Using Non-grain Biomass Sorghum saccharatum as a Substrate
Olena O. Tigunova1, *, Dzhamal B. Rakhmetov2, Yaroslav B. Blume1, Sergiy M. Shulga1
Article Information
Identifiers and Pagination:
Year: 2024Volume: 18
E-location ID: e18743315284161
Publisher ID: e18743315284161
DOI: 10.2174/0118743315284161231228065512
Article History:
Received Date: 21/10/2023Revision Received Date: 07/12/2023
Acceptance Date: 13/12/2023
Electronic publication date: 08/01/2024
Collection year: 2024
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background
The global energy challenge has recently prompted biotechnological research to explore new non-food substrates of plant origin for obtaining liquid biofuels. One of the important areas of research is the process of pretreatment and further use of non-grain biomass (lignocellulose) as a substrate for bioconversion to higher alcohols.
Objective
The aim of this work was to determine the macrocomponent composition and biochemical characteristics of sweet sorghum [Sorghum saccharatum (L.) Moench], select an effective bacterial culture for fermentation of the non-grain part of sorghum biomass as a substrate for obtaining biobutanol, and elaborate the best protective medium and storage temperature for lyophilization of the producer.
Methods
This work was conducted using butanol producing strains Clostridium sp. UCM B-7570, Clostridium acetobutylicum UCM B-7407, and C. tyrobutylicum IFBG C4B from the “Collection of Microorganism Strains and Plant Lines for Agricultural and Industrial Biotechnology” of the Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine. The bacterial cultures were cultivated on the sweet sorghum biomass provided by the National Botanical Garden named after M. M. Gryshko of the National Academy of Sciences of Ukraine. A gas chromatograph was used to determine the presence of ethanol, acetone, and butanol in the cultural liquid.
Results
It has been established that the proposed improvement of the biobutanol production process made it possible to obtain 8 g/dm3 of the target product from 60 g of dry green biomass of sweet sorghum of the Energodar variety. The composition of the protective medium for drying the Clostridium sp. UCM B-7570 culture and its storage period in the lyophilic form have been optimized.
Conclusion
The obtained results demonstrate the possibility of using the biomass of different varieties of sweet sorghum as a substrate for obtaining biobutanol, and the optimized storage conditions of the Clostridium sp. UCM B-7570 culture can minimize the possibility of its degradation.
