RESEARCH ARTICLE
Phylogenetic Diversity of Ficus Species Using HAT-RAPD Markers as a Measure of Genomic Polymorphism
S. Anuntalabhochai1, W. Phromthep2, S. Sitthiphrom3, R. Chundet4, R.W. Cutler 5, *
Article Information
Identifiers and Pagination:
Year: 2008Volume: 2
First Page: 62
Last Page: 67
Publisher ID: TOASJ-2-62
DOI: 10.2174/1874331500802010062
Article History:
Received Date: 09/06/2008Revision Received Date: 20/08/2008
Acceptance Date: 21/08/2008
Electronic publication date: 9/9/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
To create a molecular marker characterization for twenty species of Ficus, eight decamer primers were used to randomly amplify these species genomic DNA producing a total of 172 distinct polymorphic band patterns. One band was chosen to be converted into the more robust sequence characterized amplified region (SCAR) marker format to provide a unique molecular marker characterization for the variety of Ficus hirta. This technique for species identification and characterization provides a morphologically independent test to verify relatedness and provide species information particularly for cases where such identification was previously untenable such as in the case of morphologically indistinguishable plant cuttings.