RESEARCH ARTICLE


Phylogenetic Diversity of Ficus Species Using HAT-RAPD Markers as a Measure of Genomic Polymorphism



S. Anuntalabhochai1, W. Phromthep2, S. Sitthiphrom3, R. Chundet4, R.W. Cutler 5, *
1 Biology Department, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
2 Department of Biology Faculty of Science and Technology, Sakon Nakhon Rajabhat University, Sakon Nakhon 47000, Thailand
3 Department of Science, Faculty of Science and Technology, Loei Rajabhat University, Loei 42000, Thailand
4 Department of Biology, Faculty of Science, Maejo University, Chiang Mai 50200, Thailand
5 Bard College, Annandale New York, 12504, USA


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Creative Commons License
Anuntalabhochai et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Bard College, Annandale New York, 12504, USA; E-mail: cutler121@gmail.com


Abstract

To create a molecular marker characterization for twenty species of Ficus, eight decamer primers were used to randomly amplify these species genomic DNA producing a total of 172 distinct polymorphic band patterns. One band was chosen to be converted into the more robust sequence characterized amplified region (SCAR) marker format to provide a unique molecular marker characterization for the variety of Ficus hirta. This technique for species identification and characterization provides a morphologically independent test to verify relatedness and provide species information particularly for cases where such identification was previously untenable such as in the case of morphologically indistinguishable plant cuttings.

Keywords: Ficus, genomics, HAT-RAPD, SCAR markers.