RESEARCH ARTICLE


Induction of Bacterial Canker Resistance in Tomato Plants Using Plant Growth Promoting Rhizobacteria



Yuliya Kolomiiets1, *, Ivan Grygoryuk1, Artur Likhanov2, Lyudmila Butsenko3, Yaroslav Blume4
1 Department of Ecobiotechnology and Biodiversity, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
2 Department of Dendrology and Parking, Institute for Evolutionary Ecology, Natl. Academy of Sciences of Ukraine, Kyiv, Ukraine
3 Department of Phytopathogenic Bacteria, Zabolotny Institute of Microbiology and Virology, Natl. Academy of Sciences of Ukraine, Kyiv, Ukraine
4 Department of Genomics and Molecular Biotechnology, Institute of Food Biotechnology and Genomics, Natl. Academy of Sciences of Ukraine, Kyiv, Ukraine


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Creative Commons License
© 2019 Kolomiiets et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at Department of Ecobiotechnology and Biodiversity, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine; Tel: +380 (044) 527-85-17; E-mail: julyja@i.ua


Abstract

Background:

By inducing the production of inhibitory allelochemicals and mechanisms of systemic resistance Plant Growth Promoting Bacteria (PGPB) help plants to cope with stresses.

Materials and Methods:

In this study cell suspensions of Bacillus subtilis, Pseudomonas fluorescens or Azotobacter chroococcum were used to test the efficacy of these PGPB in inducing resistance in tomato (Lycopersicon esculentum Mill) against Clavibacter michiganensis subsp michiganensis, a bacteria known to cause canker disease. To test this hypothesis, seedlings of Chaika variety, characterized by short growing, early-ripening, high productivity and resistance against fusarium and the C. michiganensis strain ІZ-38 isolated in Kyiv were employed.

Results and Conclusion:

The use of cell suspensions of the PGPB B. subtilis, A. chroococcum or P. fluorescens induced an increment in the resistance of tomato plants against the causative agent of bacterial canker (C. michiganensis subsp. michiganensis) by 42–50%. PGPB in fact promoted in C. michiganensis infected tomato plants: i) the accumulation of chlorophyll a and b and carotenoids; ii) the thickening of the upper and lower epidermis of leaves; iii) the deposition of biopolymers with protective properties in epidermal cells; iv) the activity of the peroxidase enzyme and v) the net productivity of photosynthesis.

Keywords: Lycopersicon esculentum Mill., Clavibacter Michiganensis subsp. Michiganensis, Resistance, Plant growth promoting bacteria, Antioxidant activity, Phenolic compounds, Anatomical parameters.