Inhibition of Mycelial Growth of Rhizoctonia Solani by Chitosan in vitro and in vivo



Sabah Rabie Mohammed1, 2, *, Elsayed Mohammed Zeitar3, Ivan Dmitrievich Eskov1
1 Plant protection Department, Faculty of Agriculture, Saratov State Vavilov Agrarian University, Saratov, 410012, Russia
2 Plant Protection Department, Faculty of Agriculture, Damanhour University, Damanhour, 22511, Egypt
3 Plant Pathology Department, Faculty of Agriculture, Damanhour University, Damanhour, 22511, Egypt

Abstract

Objective:

Evaluate the antifungal effect of chitosan against Rhizoctonia solani in vitro and the possible mechanisms of its induced activity in potato tubers to control black scurf disease.

Methods:

The in vitro influence of chitosan at different concentrations on mycelial growth of R. solani was tested by using the poisoned food technique in PDA medium. The effect of these concentrations on the development of lesion diameters in tubers inoculated with R. solani mycelium was assayed for 30 days. The concentration that showed the greatest inhibitory effect on lesion diameters was tested to assess the induced activity of defense-related enzymes in the infected tubers.

Results:

In the poisoned food technique, chitosan at 1% completely inhibited the growth of R. solani mycelium. In vivo tests showed that chitosan treatment at 0.5% effectively controlled the black scurf in tubers inoculated with R. solani mycelium. Chitosan increased the activities of defense-related enzymes such as peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) in treated tubers of tested cultivars.

Conclusion:

This work demonstrated that chitosan directly inhibited the growth of R. solani, and potentially elicited defense reaction in potato tubers.

Keywords: Chitosan, Induced resistance, Potato, Rhizoctonia solani, Defense enzymes, Black scurf, Peroxidase.


Abstract Information


Identifiers and Pagination:

Year: 2019
Volume: 13
First Page: 3
Last Page: 8
Publisher Item Identifier: BMS-TOASJ-2019-37

Article History:

Received Date: 18/07/2019
Revision Received Date: 20/09/2019
Acceptance Date: 07/10/2019
Electronic publication date: 21/11/2019
Collection year: 2019

© 2019 Mohammed et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Correspondence: Address correspondence to this author at the Plant protection Department, Faculty of Agriculture, Saratov State Vavilov Agrarian University, Saratov, 410012, Russia; Tel: +79026665260; E-mail: srabie106@gmail.com